An Acid Citrate-dextrose Solution with Low Water Volume and Low Dextrose Concentration.

The blood preservative introduced by Loutit and Mollison (1) consisting essentially of a sodium citrate-dextrose mixture of relatively low pH, has proved superior to previously employed preserving fluids. The original formula and many subsequent modifications have a rather high volume. This produces considerable dilution of the plasma proteins. The purpose of this study, begun in June 1944, was to obtain an A.C.D. solution of lower volume, so as to maintain an optimal concentration of plasma proteins, maintain or improve the capacity to preserve erythrocytes, and at the same time insure maximal stability of the resulting plasma. The first solution tested was one proposed by Rapport, as follows: Sodium citrate (trisodium dihydric) 1.66 grams Citric acid (monohydric) 0.59 gram Distilled water 100 ml.